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frozen peripheral blood mononuclear cells pbmcs  (ATCC)


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    ATCC frozen peripheral blood mononuclear cells pbmcs
    Frozen Peripheral Blood Mononuclear Cells Pbmcs, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 878 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/frozen+peripheral+blood+mononuclear+cells+pbmcs/Primary+Peripheral+Blood+Mononuclear+Cells+(PBMC)%2C+Normal%2C+Human/pm41150397-98-0-6
    Average 99 stars, based on 878 article reviews
    frozen peripheral blood mononuclear cells pbmcs - by Bioz Stars, 2026-09
    99/100 stars

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    Bioassay:

    Article Title: Advancing Pyrogen Testing for Vaccines with Inherent Pyrogenicity: Development of a Novel Reporter Cell-Based Monocyte Activation Test (MAT).
    Article Snippet: .. Frozen peripheral blood mononuclear cells (PBMCs) (ATCC, Manassas, VA, USA), THP1-Lucia-NFκB cells (InvivoGen, San Diego, CA, USA), and TLR Bioassay cells (Promega, Madison, WI, USA) were washed with Phosphate-Buffered Saline (PBS) and plated in 96-well V-bottom plates at a density of 1 × 106 cells per well. ..

    Article Title: Advancing Pyrogen Testing for Vaccines with Inherent Pyrogenicity: Development of a Novel Reporter Cell-Based Monocyte Activation Test (MAT)
    Article Snippet: .. Frozen peripheral blood mononuclear cells (PBMCs) (ATCC, Manassas, VA, USA), THP1-Lucia-NFκB cells (InvivoGen, San Diego, CA, USA), and TLR Bioassay cells (Promega, Madison, WI, USA) were washed with Phosphate-Buffered Saline (PBS) and plated in 96-well V-bottom plates at a density of 1 × 10 6 cells per well. ..

    Saline:

    Article Title: Advancing Pyrogen Testing for Vaccines with Inherent Pyrogenicity: Development of a Novel Reporter Cell-Based Monocyte Activation Test (MAT).
    Article Snippet: .. Frozen peripheral blood mononuclear cells (PBMCs) (ATCC, Manassas, VA, USA), THP1-Lucia-NFκB cells (InvivoGen, San Diego, CA, USA), and TLR Bioassay cells (Promega, Madison, WI, USA) were washed with Phosphate-Buffered Saline (PBS) and plated in 96-well V-bottom plates at a density of 1 × 106 cells per well. ..

    Article Title: Advancing Pyrogen Testing for Vaccines with Inherent Pyrogenicity: Development of a Novel Reporter Cell-Based Monocyte Activation Test (MAT)
    Article Snippet: .. Frozen peripheral blood mononuclear cells (PBMCs) (ATCC, Manassas, VA, USA), THP1-Lucia-NFκB cells (InvivoGen, San Diego, CA, USA), and TLR Bioassay cells (Promega, Madison, WI, USA) were washed with Phosphate-Buffered Saline (PBS) and plated in 96-well V-bottom plates at a density of 1 × 10 6 cells per well. ..



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    a diABZI-i inhibits cGAMP-induced IFNβ in <t>PBMCs</t> ( n = 3 | 1 donor) and diABZI-a1 induces IFNβ in PBMCs ( n = 3 | 3 donors, representative donor displayed). Data represented as mean with SD in graph. Source data are provided as a Source Data file. b Superposition of crystal structures (side view) of STING bound to diABZI-a1 (gray) and diABZI-i (purple). c diABZI-a1 and diABZI-i superposition over the STING binding pocket. d Cartoon depicting apical wing distance and beta sheet lid between STING monomers. e Superposition of crystal structures (top view) of STING bound to diABZI-a1 (gray) and diABZI-i (purple). f THIQi inhibits cGAMP-induced IFNβ in PBMCs ( n = 3 | 1 donor). Data represented as mean with SD in graph. Source data are provided as a Source Data file. g Crystal structure overlay (top view) of STING bound to THIQi (orange) and diABZI-i (purple). h Apical wing distance measurements from structures in this study and reference structures collected from the PDB.
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    a diABZI-i inhibits cGAMP-induced IFNβ in PBMCs ( n = 3 | 1 donor) and diABZI-a1 induces IFNβ in PBMCs ( n = 3 | 3 donors, representative donor displayed). Data represented as mean with SD in graph. Source data are provided as a Source Data file. b Superposition of crystal structures (side view) of STING bound to diABZI-a1 (gray) and diABZI-i (purple). c diABZI-a1 and diABZI-i superposition over the STING binding pocket. d Cartoon depicting apical wing distance and beta sheet lid between STING monomers. e Superposition of crystal structures (top view) of STING bound to diABZI-a1 (gray) and diABZI-i (purple). f THIQi inhibits cGAMP-induced IFNβ in PBMCs ( n = 3 | 1 donor). Data represented as mean with SD in graph. Source data are provided as a Source Data file. g Crystal structure overlay (top view) of STING bound to THIQi (orange) and diABZI-i (purple). h Apical wing distance measurements from structures in this study and reference structures collected from the PDB.

    Journal: Nature Communications

    Article Title: Orthosteric STING inhibition elucidates molecular correction of SAVI STING

    doi: 10.1038/s41467-025-60632-5

    Figure Lengend Snippet: a diABZI-i inhibits cGAMP-induced IFNβ in PBMCs ( n = 3 | 1 donor) and diABZI-a1 induces IFNβ in PBMCs ( n = 3 | 3 donors, representative donor displayed). Data represented as mean with SD in graph. Source data are provided as a Source Data file. b Superposition of crystal structures (side view) of STING bound to diABZI-a1 (gray) and diABZI-i (purple). c diABZI-a1 and diABZI-i superposition over the STING binding pocket. d Cartoon depicting apical wing distance and beta sheet lid between STING monomers. e Superposition of crystal structures (top view) of STING bound to diABZI-a1 (gray) and diABZI-i (purple). f THIQi inhibits cGAMP-induced IFNβ in PBMCs ( n = 3 | 1 donor). Data represented as mean with SD in graph. Source data are provided as a Source Data file. g Crystal structure overlay (top view) of STING bound to THIQi (orange) and diABZI-i (purple). h Apical wing distance measurements from structures in this study and reference structures collected from the PDB.

    Article Snippet: Frozen human peripheral blood mononuclear cells (PBMCs, BioIVT) are gently thawed, suspended in complete medium [AIMV, 10% heat-inactivated FBS, 1% antibiotic-antimycotic, 1% sodium pyruvate, 1% MEM non-essential amino acids; ThermoFisher] and concentrated by low-speed centrifugation (400 × g, ambient temperature, five minutes).

    Techniques: Binding Assay